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KMID : 0811720100140020059
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Korean Journal of Physiology & Pharmacology
2010 Volume.14 No. 2 p.59 ~ p.69
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Are Spinal GABAergic Elements Related to the Manifestation of Neuropathic Pain in Rat?
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Lee Jae-Hee
Back Seung-Keun
Lim Eun-Jeong
Cho Gyu-Chong
Kim Myung-Ah
Kim Hee-Jin
Lee Min-Hee
Na Heung-Sik
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Abstract
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Impairment in spinal inhibition caused by quantitative alteration of GABAergic elements following peripheral nerve injury has been postulated to mediate neuropathic pain. In the present study, we tested whether neuropathic pain could be induced or reversed by pharmacologically modulating spinal GABAergic activity, and whether quantitative alteration of spinal GABAergic elements after peripheral nerve injury was related to the impairment of GABAergic inhibition or neuropathic pain. To these aims, we first analyzed the pain behaviors following the spinal administration of GABA antagonists (1?g bicuculline/rat and 5?g phaclofen/rat), agonists (1?g muscimol/rat and 0.5?g baclofen/rat) or GABA transporter (GAT) inhibitors (20?g NNC-711/rat and 1?g SNAP-5114/rat) into naive or neuropathic animals. Then, using Western blotting, PCR or immunohistochemistry, we compared the quantities of spinal GABA, its synthesizing enzymes (GAD65, 67) and its receptors (GABAA and GABAB) and transporters (GAT-1, and -3) between two groups of rats with different severity of neuropathic pain following partial injury of tail-innervating nerves; the allodynic and non-allodynic groups. Intrathecal administration of GABA antagonists markedly lowered tail-withdrawal threshold in naive animals, and GABA agonists or GAT inhibitors significantly attenuated neuropathic pain in nerve-injured animals. However, any quantitative changes in spinal GABAergic elements were not observed in both the allodynic and non-allodynic groups. These results suggest that although the impairment in spinal GABAergic inhibition may play a role in mediation of neuropathic pain, it is not accomplished by the quantitative change in spinal elements for GABAergic inhibition and therefore these elements are not related to the generation of neuropathic pain following peripheral nerve injury.
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KEYWORD
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GABA, GAD65, GAD67, GAT-1, GAT-3, Peripheral nerve injury, Neuropathic pain
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